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Fast qPCR detection system for high-quality data with smart protocols, and robust durability

Quick Set up and Plate Layout Confirmations

Each well is easily labeled with three identifiers:

  • Well type,
  • Sample ID, and
  • Dye/target.

The identifiers are used for data analysis and easy visualization of the amplification and standard curves.

View Amplification Trace Individually by Target or Sample

Easily view all of your data at once or select specific wells/targets to view a subset of your data. Cielo’s software allows you to easily adjust threshold values by dragging the threshold line, or typing in the threshold value.

You can visualize amplification curves in linear or log view, and change the color of the amplification curves to easily distinguish the data by Sample ID or target.

Quickly Understand Data

Quickly review Cq values, and assess data uniformity and separation between dilutions.

The wells shown here are colored in a 2D Sample Heat Map based on Cq value, with the lowest Cq values in green and highest Cq values in red.

Optical Design Eliminates the Need for ROX

Well-based excitation and color-specific LED light sources ensure the excitation efficiency in every well for every channel is the same.

Coupled with scanning technology that collects multiple data points from each well, the Cielo’s well-based optical design eliminates the need for normalization dyes, such as ROX. Rather than a single, stationery light source and detector system, the Cielo utilizes 16-well simultaneous excitation and signal detection that ensures uniform data acquisition.